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Eurokat – valuable for food analysis

Eurokat high-performance polymer phases were specially developed for the separation of organic acids, carbohydrates, alcohols and even complex mixtures of these compounds.

Eurokat is a sulfonated cross-linked styrene-divinylbenzene copolymer available in several ionic forms (H, Ca, Pb and Ag). This particular cation exchanger is characterized by an outstanding density of functional groups. This makes it an ideal choice for your ion exclusion, size exclusion and ligand exchange chromatography.

Application areas

Four different ionic species are available. Eurokat H is specially designed for the analysis of organic acids and even complex mixtures of acids, sugars and alcohols as well as sugar alcohols. Eurokat Ca and Eurokat Pb are the phases of choice for analysis of smaller carbohydrates (DP < 4), Eurokat Ag is applicable for the separation of sugar oligomers. Eurokat can also be used for the determination of biochemically important molecules such as sugars, artificial sweeteners or acidic metabolic and degradation products. Other application areas include analysis of:

  • carbohydrates and acids in fruit beverages and soft drinks
  • sugar substitutes
  • food preservatives
  • dairy products
  • intermediate acids of the Krebs cycle
  • urine (uric acid, hippuric acid)
  • monitoring of fermentation processes

Stability

Eurokat polymer columns are extremely stable over the whole pH range. This is one striking advantage which you get when compared with silica-based phases which have a limited lifetime at pH extremes. This is especially in the higher pH range. Most importantly, Eurokat phases show extraordinary column lifetime stability and are not affected by aqueous solvents.

Choice of chromatographic conditions

Outstanding separation results are achieved isocratically with the help of fresh double-distilled water for Pb or Ca, or Ag type Eurokat columns, while Eurokat H columns perform best when using inorganic acids. Such aqueous eluents produce no harmful waste as with organic solvents such as acetonitrile which necessitate expensive disposal.

Choice of chromatographic conditions

The Eurokat stationary phase is designed to be applicable to a wide range of diverse chromatographic conditions. All Eurokat columns can be used at temperatures up to 90 °C under diverse pH conditions (e.g. using 0.001– 0.1 N H2SO4). For example, the best separation of sugars can be achieved using an acidic aqueous mobile phase at a temperature between 60 to 90 °C. To extend separation performance, it is possible to connect up to three columns of Eurokat in series.

KNAUER column hardware

We design and manufacture HPLC column hardware under strict quality control. A specially treated inner surface ensures consistent column packing and high column stability. Eurokat columns are available in lengths of 30, 120, and 300 mm. The 30 mm version is recommended as a guard column.

Applications

Separation of a mixture of organic acids, alcohols and sugars.

Eurokat H (300 x 8 mm)

Eluent: 0.01N H2SO4, Temp.: 75 °C, Flow: 0.5 ml/min, Detection: RI

Sample: 1. isomalt, 2. lactate, 3. gluconic acid, 4. mannite, 5. xylite, 6. threitole,

  1. glycerine, 8. acetic acid, 9. ethylene glycol, 10. 1,2-propanediol, 11. methanol, 12. Ethanol

Eurokat Pb (300 x 8 mm)

Eluent: water, Temp.: 75 °C, Flow: 0.5 ml/min, Detection: RI

Sample: 1. sucrose, 2. glucose, 3. galactose, 4. fructose, 5. mannite, 6. Xylite

Eurokat Ca (300 x 8 mm)

Eluent: water, Temp.: 75 °C, Flow: 0.5 ml/min, Detection: RI

Sample: 1. lactose, 2. glucose, 3. galactose, 4. arabinose, 5. mannite, 6. Xylite

Applications

Separation of sugar oligomers.

Eurokat Ag (300 x 8 mm)

Eluent: water, Temp.: 75 °C, Flow: 0.5 ml/min, Detection: RI

Sample: 1. maltohexaose, 2. maltopentaose, 3. maltotetraose, 4. maltotriose, 5. maltose, 6. glucose, 7. fructose

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